University of Illinois Cancer Center members are among the authors of the study, “Correlative Multiscale 3D Imaging of Mouse Primary and Metastatic Tumors by Sequential Light Sheet and Confocal Fluorescence Microscopy,” published in the journal iScience.
Cancer Center member Steve Seung-Young Lee, PhD, Assistant Professor in the Department of Pharmaceutical Sciences at the Retzky College of Pharmacy at the University of Illinois Chicago (UIC) is the corresponding author. The other Cancer Center authors are Ekrem Emrah Er, PhD, Assistant Professor in the Department of Physiology and Biophysics at the University of Illinois College of Medicine at UIC, and Zongmin Zhao, PhD, Assistant Professor in the Department of Pharmaceutical Sciences at the College of Pharmacy.
The study’s summary is excerpted below:
“Three-dimensional (3D) optical microscopy permits in situ interrogation of the tumor microenvironment (TME) in volumetric tumors for research while light sheet and confocal fluorescence microscopy are often used to achieve macroscopic and microscopic 3D images of tissues, respectively. Although each technique offers distinct fields of view (FOVs) and spatial resolution, the combination of the two to obtain correlative multiscale 3D images from the same tumor tissues has not yet been explored. We established a workflow that enables the tracking and 3D imaging of region of interests (ROIs) within tumor tissues through sequential light sheet and confocal fluorescence microscopy. This approach allowed for quantitative 3D spatial analysis of the immune response in the TME at multiple spatial scales and facilitated the direct localization of a metastatic lesion within a mouse brain. Our method offers an approach for correlative multiscale 3D optical microscopy with the potential to provide new insights into comprehensive research in disease mechanism or drug response.”
Other authors affiliated with UIC are Zaijie J. Wang, PhD, UIC Distinguished Professor and Professor of Pharmacology and Pharmaceutics in the College of Pharmacy; Jingtian Zheng, PhD, former Lee Lab member; Yi-Chien Wu, PhD, Graduate Research and Teaching Assistant in the Lee Lab; Xiaoying Cai, PhD, student and Lee lab member; Philana Phan, Graduate Student and Zhao lab member; Meghna Gill, Graduate Student and Wang lab member.